T and Biocomma, and the elution volume was 80 L. Meanwhile, extracted plasmid pUC19 from 10 mL overnight E.coli (DH5a) using Biocomma HP50, and the elution volume was 100 L. Then analyze by electrophoresis on a 1% agarose gel.
Lane 1: Column from company A
Lane 2: Column from company T
Lane 3: Biocomma NP30
Lane 4: Biocomma HP50
Figure 1: Plasmid DNA extracted using various columns
Experimental overview
Table 1: The yield of plasmid DNA extracted using various columns